Association between microsatellite markers and the resistance gene to common bacterial blight in F 2:4 families of Phaseolus vulgaris L.
Abstract
The incidence of disease is one of the factors limiting the production of black bean (Phaseolus vulgaris L.). The common bacterial blight of bean caused by Xanthomonas phaseoli (Xph) is the most important crop bacterial disease because reduces grain yield by 45% and also affects the quality of the seed. It is necessary to incorporate resistance genes through crossing programs. The object of this study was to verify the association between type microsatellite molecular markers (SSR) and the resistance gene to common bacterial blight in families F 2:4 of black bean. The molecular evaluation was performed usig the molecular amplification primers PV-ctt001, PV-atgc001, BM-140, BM-156, and BM-202, that has previously showed a possible association with resistance. For amplification of SSR, the standardized in the conditions in the molecular genetics laboratory were used. Expected segregation of F 2:4 families for each SSR was verified using MapDisto Genetics V 6.3 program. BM-140 behaved as a monomorphic marker and VPV-ctt001, as an independent locus, so it was not possible to differentiate between resistant families using these markers. Significant association between SSR: BM-156, BM-202 and resistance controlled by a dominant gene in families F2:4 was found Xph marker and PV-atgc001 as an independent locus used. Assisted selection to the “b” allele using these SSR will be useful to identify plants carrying the resistance in black bean breeding programs
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References
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Copyright (c) 2014 Rossmary Castañeda, Catalina Ramis, Delis P´érez
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