Isoenzymatic characterization of isolated from Trichoderma spp

Abstract

Recently it has been shown that the techniques are highly isozyme support traditional morphological criteria clearly delineated within and between species, as well as the relationship between pathogenic and electrophoretic tests among these microorganisms. The research aimed to implement the use of isozyme technique to characterize the genetic variability of isolates of Trichoderma spp. Were used Trichoderma isolates including T. harzianum, T. koningiopsi, T. longibrachiatum and T. atroviride from southern Aragua State and northern Guárico State. Isozyme twelve systems were evaluated: alpha and beta esterase, alcohol dehydrogenase, malate dehydrogenase, alkaline phosphatase, acid phosphatase, peroxidase, glutamate oxaloacetate transaminase, isocitrate dehydrogenase, malic enzyme, glucofosfo isomerase, glutamate dehydrogenase. A cluster was performed using as a metric distance (the distance of Jaccard similarity), the data is analyzed using Past® Version 1.55. According to the isoenzyme electrophoretic patterns, there is variability in the isolates, this due to the formation of four groups corresponding to the locations of collections and not evaluated Trichoderma species. Cluster analysis (cluster) revealed the formation of 4 groups. I and II, formed by isolates from Aragua State. The III, composed of the isolated from production areas Guárico and Aragua States, consisting of Group IV isolates from Guárico State.