Molecular diagnosis of Xanthomonas albilineans (Ashby) Dowson in Venezuela, the causal agent of leaf scald of sugarcane
Abstract
Leaf scald caused by Xanthomonas albilineans, is one of the most important diseases in sugarcane, Saccharum sp., which causes serious economical losses estimated between90-100%. The use of PCR as a diagnostic tool for plant pathogens has increased because of its speed, sensitivity, and specificity. Given the importance of leaf scald in Venezuela, in this work we carried out the diagnosis of X. albilineans from symptomatic sugarcane plants, using microbiological, biochemical and molecular methods. Bacterial isolates were obtained using different methods of purification. These isolates were then subjected to phenotypic and biochemical analysis, PCR with specific primers and ERIc PCR. With the method of macerated leaves, 50 isolates were obtained, out of which 14 were selected for biochemical tests. From these 14 isolates, only three strains were identified as X. albilineans by microbiological and biochemical tests. These results were confirmed by PCR by obtaining the expected band of 360 bp using specific primers. Finally, the ERIc-PCR banding patterns obtained for the isolates identified as X. albilineans were found to be different from those described by other authors in other regions. This work represents the first genotyping assay of isolates of X. albilineans in Venezuela.
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Copyright (c) 2011 Beatriz Alvez, Jeismar Carballo, Guillermina Alonso, Maira Oropeza
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