Capacity atmospheric nitrogen fixation of native strains of venezuelan ecosystems
Abstract
The low efficiency of industrial nitrogen fertilizers, as well as the high energetic and environmental costs, make necessarily generate low impact technologies like biofertilizers based on atmospheric nitrogen-fixing bacteria to substitute partially or totally the industrial sources. The population of native microorganisms and its capacity of nitrogen fixation (CFN) were estimated using agitation-fermentation tests by bioproduct agitation during 72 h in the laboratory. Simultaneously strains were inoculated in the culture medium Ashby and Dimargon®-M and shaking daily by six hours for 15 d. A randomized design with three repetitions was used. Nine strains of atmospheric nitrogen-fixing free life bacteria (FNVL), were assessed, divided into two groups. Group 1 with four strains: FNGMBar; FNMGSRt4f; FNMGSRt2f; FNMGSRt1f, isolated from treatments evaluated in a field with agroecological principles. And the group 2 with five strains: 9, 12, 17, T5 and B1 belong to the National Strain Collection of INIA-CENIAP. The nitrogen (N) produced by each strain and its equivalent in kg ha-1 was significantly (P<0.05) different between treatments evaluated. All strains were pre-selected by its CFN and by maintaining high populations, 1010 and 1013 CFU ml-1 in Ashby and Dimargon®-M mediums, respectively, ensuring high concentration and low doses of application. Group 1 could provide the soil between 46 and 50 and group 2 between 39 and 48 kg N ha-1, respectively, in crops of socioproductive interest.
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References
• Constitución de la República Bolivariana de Venezuela. 1999. 99 p. Disponible en: https://bit.ly/2UpkC8p
• Day, J. H. and J. Dobereiner. 1976. Physiological aspects of N2-fixation by Spirillum from Digitaria roots. Soil. Biol. Biochem. 8:45-50.
• Dibut, B. y R. Martínez-Viera. 2001. DIMARGON-M, medio de cultivo para la fabricación industrial de biofertilizantes y bioestimuladores microbianos. Cultivos Tropicales, 36:45-52.
• Dobbelaere, S., A. Croonenborghs and A. This. 2001. Azospirillum. Australian Journal Plant Physiology, 28:871-879.
• Dyson, T. 1996. Population and Foods. Global Trends and Future Prospects. Global Environmental Programme Rutledge. 45 pp.
• España, M., E. Cabrera and M. López. 2006. Study of nitrogen fixation by tropical legumes in acid soil from Venezuelan savannas using 15N. Interciencia. 31:197-201.
• Galloway, J. N. 1998. The global nitrogen cycle: changes and consequences. Environmental Pollution, 102(S1):15-24.
• Galloway, J. N., J. D. Aber, J. W. Erisman, S. P. Seitzinger, R. W. Howarth, E. B. Cowling and B. J. Cosby. 2003. The Nitrogen Cascade. BioScience, 53(4):341-356.
• Kjeldahl, J. 1983. A new method for the determination of nitrogen organic matter. 2. Analytical Chemistry, 22,366.
• Leaungvutiviroj, C., P. Ruangphisarn and P. Hansanimitkul. 2010. Development of a new biofertilizer with a high capacity for N2 fixation, phosphate and potassium solubilization and auxin production. Biosciencie Biotechnology and Biochemistry, 74(5):1.098-1.101.
• Ley de Salud Agrícola Integral. 2008. Decreto N° 6129 con Rango, Valor y Fuerza de Ley de Salud Agrícola Integral. Gaceta Oficial 5890 extraordinaria de la Rep. Bol. Vzla.
• López, M., R. Martínez-Viera, M. Brossard, A. Bolívar, N. Alfonzo, A. Alba y H. Pereira. 2008. Efecto de biofertilizantes bacterianos sobre el crecimiento de un cultivar de maíz en dos suelos contrastantes venezolanos. Agronomía Trop. 58(4):391-401.
• López, M., B. Rodríguez y A. Bolívar. 2009. Estrategias del Estado venezolano para consolidar el uso de biofertilizantes en la agricultura. Simposio sobre biofertilizantes, riesgos y ventajas. XVIII. Congreso Venezolano de la Ciencia del Suelo. Santa Bárbara del Zulia, del 09 al 13 de marzo de 2009. 3 p.
• López, M. 2010. Manejo agroecológico del sistema sorgo -frijol, efecto sobre la fertilidad del suelo y microorganismos con potencial para biofertilizar agroecosistemas venezolanos. Tesis de doctorado en Ciencia del Suelo. Postgrado en Ciencias del Suelo. Maracay, Ven. Universidad Central de Venezuela. Facultad de Agronomía. 211p.
• Martínez-Cruz, A., T. Bach, A. Delgado y V. Martínez. 1993. Fosforina, un nuevo biopreparado para la Agricultura. VII Forum de Ciencia y Técnica. La Habana, 68 p.
• Martínez-Viera, R., M. López, M. Brossard, G. Tejeda, H. Pereira, C. Parra, J. Rodríguez y A. Alba. 2006. Procedimientos para el estudio y fabricación de Biofertilizantes Bacterianos. Maracay. Venezuela. Instituto Nacional de Investigaciones Agrícolas. 88 p. (Serie B No 11).
• McCown, R. L., A. L. Cogle, A. P. Ockwell and T. G. Reeves. 1988. Nitrogen supply to cereals in legume ley systems under pressure. In: Advances in Nitrogen Cycling in Agricultural Ecosystems. C. A. B. International, Wallingford. Ed. J. R. Wilson. 292-314 pp.
• Organización de Naciones Unidas para la Agricultura y la Alimentación (FAO). 2004. Pérdida de la biodiversidad fitogenética. Disponible en: https://bit.ly/2xse5kn
• Peoples, M. B and E. T. Craswell.1992.Biological nitrogen fixation: Investments, expectations and contributions to agriculture. Plan and Soil, 141:13-39.
• Thompson, L. M. y F. R. Troeh. 1982. Los suelos y su fertilidad. Barcelona España. Editorial Reverté, S. A. 387 p.
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