Characterization of genetic variability using RAPDs markers, in a group of native and commercial genotypes of bean in Venezuela

  • Margaret Gutiérrez Mulas Instituto Nacional de Investigaciones Agrícolas (INIA), Centro Nacional de Investigaciones Agropecuarias (CENIAP), Maracay. Venezuela.
  • Carmen Amalia Rincón Instituto Nacional de Investigaciones Agrícolas (INIA), Centro Nacional de Investigaciones Agropecuarias (CENIAP), Maracay. Venezuela.
Keywords: Phaseolus vulgaris, landraces, RAPDs, genetic variability

Abstract

To characterize the genetic variability of a group of native genotypes, collected from small farmers' production systems and commercial varieties of the germplasm bank accessions at Centro Nacional de Investigaciones Agropecuarias (CENIAP), Venezuela, RAPDs molecular markers were used. primers of the series M and Y, (opM16 and OPY17), were useful for their efficiency in the separation between cultivated and native genotypes. primers opM-16, opY-07 opY-03 opY-04, opM-20, opA-07 and opY-17 produced 47 reproducible amplification fragments classifying native and commercial genotypes into six groups, and separating the commercial control and one enhanced line from the other germplasm evaluated. Those groups are useful for the development of a breeding program, inclu- ding broadening the genetic base of Venezuelan cultivars, to improve black beans cultivars, through crosses to Tacarigua, the group I, with the black genotypes 19 and 13, which, in another experiment with water deficit assessment, reached arithmetic and geometric average yields far superior to both commercial controls and were classified in group VI, located at 0.75 ultrametric units (UUM). Also, through crosses of the improved line 13, group VI, with black seed genotypes 6 and 12, both with high yields and classified in Group III, at 0.75 UUM. It is also advisable to make the crossing with genotype 12, characterized by a low drought susceptibility index. For breeding genotypes of nonblack seeds, it is advisable the crossing of pink seed genotype 10, group II, with the white seed genotype 2 classified in group V, at 0.60 UUM. 

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References

• Bannerot. J. 1999. A genetic map of common bean to localize specific resistance genes against anthracnose. plant genome II conference “International plant genome conference”. Thematic items Fifth PHASElIEU Workshop. Town & country conference center, San Diego, CA. Genome 37:915-924
• Beebe, S. E., I. Ochoa, P. Skroch, J. Nienhuis and J. Tivang. 1995. genetic Diversity among common bean breeding lines developed for central América. Crop Sci. 35(5):1 178-1 183.
• Beebe, S., P. W. Skroch, J. Thome, M. C. Duque, F. Pedraza and J. Nienhuis. 2000. Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysisi of RAPD. Crop Sci., 40(1):264-273.
• Beebe, S., J. Rengifo, E. Gaitan, M. C. Duque and J. Thome. 2001. Diversity and origin of Andean landraces of common Bean. crop Science. 41:854-862.
• Berthaud, J. 1997. Strategies for conservation of genetic resources in relation with their utilization. Euphytica.96:1-12.
• Brucher, O. B. and H. Brucher. 1976. The South American wild bean (Phaseolus aborigieus Burk.) as ancestor of the common bean. Economic Botany. 30(3):257-272.
• Constabel, E. C., T. E. Michaels, P. H. Goodwin, J. E. Mayer and M. A. Pastor. 1996. Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a verdín program. Euphytica. 90:129-135.
• Debouck, D. G., O. Toro, O. M. paredes, W. C. Johnson and P. Gepts. 1993. genetic diversity and ecological distribution of Phaseolus vulgaris (Fabaceae) in northwestern South America. Economic Botany, 47(4):408-423.
• Dice, l. R. 1945. Measures of the amount of ecologic association between species. Ecology. 26:297-302.
• Faleiro, F. G., W. S. Vinhadelli, V. A. Ragagnin, R. X. correa, M. A. Moreira and E. G. de Barros. 2000. RAPD markers linked to a block of genes conferring rust resistance to the common bean. Genet. Mol. Biol. 23(2):399-402.
• Fall, A. l., P. F. Byrne, G. Jung, D. P Coyne, M. A. Brick and H. F. Schwartz. 2001. Detection and mapping of a major locus for Fusarium wilt resistance in common bean. Crop Science, 41:1494-1498.
• Ferreira Da Silva, G., J. B. Dos Santos and M. A. Patto. 2003. Identification of SSR and RApD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAl 550. Genetics and Molecular Biology. 26(4):459-463.
• Ferreira, C. F., A. Borém, g. A. Carvalho, S. Nietsche, T. J. Paula Jr., E. G. Barros and M. A. Moreira. 2000. Inheritance of angular leaf spot resistance in common bean and identification of a RAPD marker linked to a resistance gene. crop Science. 40:1 130-1 133.
• Fofana, B. J. P., J. P. Baudion and P. Du Jardin. 1998.Molecular phylogeny in the genus Phaseolus. 3rd European conference on grain legumes. oportunities for high quality, healthy and added-value crops to meet European demands. Valladolid, España. 214-215 pp.
• Fofana, B., X. Vekemans, P. Du Jardin and J. P. Baudion. 1997. Genetic Diversity in lima Bean (Phaseolus lunatus) as reveled by RApD markers. Euphytica,95:157-165.
• Freyre, R., R. Rios; l. Guzman, D. G. Debouck and P. Gepts. 1996. Ecogeographic distribution of Phaseolus spp. (Fabaceae) in Bolivia. Economic Botany. 50(2):195-216.
• Gutiérrez, M. 2009. Caracterización ecofisiológica y molecular de materiales locales de caraota (Phaseolus vulgaris l.) colectados en Venezuela, ante condiciones de déficit hídrico. Tesis de grado para optar al título de Doctora en ciencias Agrícolas. Universidad Central de Venezuela. 69 p.
• Haley, S. D., P. N. Miklas, l. Afanador and J. D. Kelly. 1994. Random amplified polymorphic DNA (RAPD) marker variaility between and within gene pools of common bean. J. Amer. Soc. Hort. Sci. 119:122-125.
• Jaffé, W. G. and G. Karl. 1959. Purificatión of a toxic phytohaemagglutinin from Black Beans (Phaseolus vulgaris). Nature. 183:1 329-1 330.
• Jaffé, W. G. and K. Hannig. 1965. Fractionation of proteins from kidney beans (Phaseolus vulgaris). Arch. Biochem. Biophys. 109:80-91.
• Jaffé, W. G. y O. Brucher. 1968. La Presencia de fitohemaglutinina en Phaseolus aborigineus (Burk) y su identidad con la de Phaseolus vulgaris l. como argumento quimiotaxonómico de la íntima relación de estas dos especies. Rev. Acta Científica Venezolana. 19:20.
• Karp, A. 2002. The new genetic era: will it help us in managing genetic diversity. In managing plant genetic diversity. Edited by J. M. M. Engels, V. Ramanatha Rao, A. H. D. Brown and M. T, Jackson. CABI Publishing. 487 p.
• Kaplan, l. 1965. Archeology and domestication in American Phaseolus (Bean). Econ. Bot. (19):358-368.
• Koenig, R. l., S. P. Singh and P. Gepts. 1990. Novel phaseolin Types in wild and cultivated common bean (Phaseolus vulgaris, Fabaceae). Economic Botany, 44(1):50-60.
• Koinange, E. M., S. Singh and P. Gepts. 1996. genetics control of the domestication syndrome in common bean. crop Sci, 36:1 037-1 045.
• lowe, A. J., O. Hanotte and l. Guarino. 1996. Standardization of molecular genetic techniques for the characterization of germplasm collections: the case of random amplified polymorphic DNA (RAPD). Plant genetic Resources Newsletter, 107:50-54.
• Mailer, R. D., R. Scarth and B. Fristensky. 1994. Discrimination among cultivars of rapeseed (Brassica napus) using DNA polymorphisms amplified from arbitary primers. Theoretical and Applied genetics, 87:697-704.
• Métias, I., C. Aubry, B. Hamon, R. Jalouzot and D. Peltier. 2000. Description and analysis of genetic diversity between commercial bean lines (Phaseolus vulgaris). Theoretical and Applied Genetics, 101(8):1 207-1 214.
• Miklas, P. N., W. C. Johnson and R. Delorme and P. gepts. 2001. QTlconditioning physiological Resistance and Avoidance to White Mold in Dry Bean. Science. 41:309-315.
• Mitrick, J. A., J. Nienhnis, P. Hinrichsen and C. Muñoz. 1995. RApID Analysis of phaseolus vulgaris lan- drace from chile. Department of Biological Science Northern Illinois University De Kalb, Department of Horticulture, University of Wisconsin, Madison, Instituto Nacional de Investigaciones Agropecuarias, Santiago, chile, Plant genome III, January 1995.
• Nowosielski, J., W. Podyma and D. Nowosielska. 2002. Molecular research on the genetic diversity of polish varieties and landraces of Phaseolus coccineus l. and Phaseolus vulgaris l. using the RApD and AFlp methods. cell Mol Biol lett, 7(2B):753-762.
• Park, Soon O., D. P. Coyne, J. R. Steadman and P. W. Skroch. 2001. Mapping of QTl for Resistance Mold Disease in common Bean. Crop Science, 41:1 253-1 262.
• Quirós, C. F., J. Hu, P. This, A. M. Chevre and M. Delseny. 1991. Development and chromosomal localization of genome-specific markers by the polymerase chain reaction. Theoretical and Applied genetics, 82:627-632.
• Sánchez, M., S. Hernández, P. Guzman and J. Simpson. 1995. A simple protocol for the isolation of High Molecular Weight DNA Molecular from common Bean (P. vulgaris). Department of genetic Engineering. centro de Investigaciones y Estudios Avanzados (CINVESTAV), Unidad Irapuato Apdo postal 629, Irapuato México.
• Sing, S. P., P. Gepts and D. G. Debouck. 1991a. Races of common Bean (Phaseolus vulgaris, Fabaceae). Economy Botanic, 45(3):79-396.
• Sing, S. P., J. A. Gutiérrez, A. Molina, C. Urrea and P. Gepts. 1991b. Genetic Diversity in common Bean: II. Marked-Based Analysis of Morphological and Agronomics Traits. crop Sciece, 31:23-29.
• Skroch paul, W., J. Nienhuis, S. Beebe, J. Thome and F. Pedraza. 1998. Comparison of Mexican Common Bean (Phaseolus vulgaris l.) core and Reserve Germplasm Collections. Crop Science, 38:488-496.
• Vekemans, X., O. Hardy, B. Berken, B. Fofana and J. Baudoin. 1998. Use of PCR-RFLP on chloroplast DNA to investigate phylogenetic realtionships in the genus phaseolus. Biotechnologie, Agronomie Societe et Environnement. 2(2):128-134.
• Voysest, O. 2000. Mejoramiento genético del frijol (Phaseolus vulgaris L.) legado de variedades de América latina 1930-1999. centro Internacional de Agricultura Trop. Cali Colombia. 195 p.
• Welsh, J. and M. Mcclelland. 1990. Fingerprinting genomes using pcR with arbitrary primers. Nucleic Acids Research, 18(24):7 213-7 218
• Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22):6 531-6 535.
• Yang, X. and C. Quiros. 1993. Identification and classification of celery cultivars with RAPD markers. Theoretical and Applied Genetics, 86:205-212.
• Zambrano, A. Y., J. R. Demey, G. Martínez, F. Fuenmayor, Z. Gutiérrez, G. Saldaña y M. Torrealba. 2002. Método rápido, económico y confiable de mini preparación de ADN para amplificaciones por RAPD en bancos de germoplasma. Agronomía Trop.,52(2):235-243.
Published
2011-03-31
How to Cite
Gutiérrez Mulas, M., & Rincón, C. A. (2011). Characterization of genetic variability using RAPDs markers, in a group of native and commercial genotypes of bean in Venezuela. Agronomía Tropical, 61(1), 73-83. Retrieved from http://publicaciones.inia.gob.ve/index.php/agronomiatropical/article/view/308
Section
Original research article