Evaluation of different sources of carbohydrates and medium of support for the in vitro multiplication of rootstock tolerant to citrus tristeza virus
Keywords:
Rootstocks, citrus, tissue culture, sugars, vermiculite
Abstract
The use of the sugars xylose, fructose, and 10X20F, as substitutes for sucrose, was evaluated in terms of effects on the number and height of shoots, the number of leaves, roots per shoot, and rooting shoots in rootstocks of the Citrus “tristeza” virus (CTV) resistant lemon varieties Citrus volkameriana, Citrumelo swingle and C-35. The explants were cultured in vermiculite and in agar in order to concurrently compare the effects of these different culture media. An average of two shoots per explant was observed in all three species in all three carbohydrate treatments. Shoot height, number of leaves per shoot, and number of roots in both culture media, were not found to be significantly different (P<0.05). A higher percentage of rooting shoots was observed in the sucrose and 10X20F treatments in both culture media (99-100%). Treatments utilizing xylose and fructose showed a response of 75-89% rooting. The results showed that neither carbohydrate source nor culture medium had a significant effect on in vitro multiplication, but that shoot height was higher in the rootstock of C. volkameriana.
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References
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• Chen, Y., S. Lin, P. Duguid, E. Dribnenki and E. Kenaschuck. 2003. Effect of sucrose concentration on elongation of shoots from flax anther culture. Plant Cell Tissue and Organ Culture. 68:49-55.
• Chu, E. P. and R. C. L. Figueiredo-Ribeiro. 2002. Grow and carbohydrate changes in shoot cultures of dioscorea species as influenced by photoperiod, exogenous sucrose and cytokinin concentrations. Plant Cell Tissue and Organ Culture. 70:241-249.
• FAO. 2005. Base de datos de FAOSTAT. Food and agriculture organization. https://bit.ly/2EHapil. Food and agriculture organization. Consultado el 15 de abril de 2006.
• Faria R., T., F. N. Rodriguez, L. V. R. Oliveira and C. Muller. 2004. In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations. Horticultura Brasileira, Brasilia. 22(4):780-783.
• García, J., L. J. Troncoso, R. Sarmiento and A. Troncoso. 2002. Influence of carbon source and concentration on the in vitro development of olive zygotic embryos and explants raised from them. Plant Cell Tissue and Organ Culture. 69:95-109.
• Gollagunta, V. J., W. Adelberg, R. Rieck and N. Rajapakse. 2004. Sucrose concentration in liquid media affects soluble carbohydrates, biomass and storage quality of micropropagated hosta. Plant Cell Tissue and Organ Culture. 77:125-131.
• Gómez, M. P. and J. Segura. 1995. Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree Physiology 15(9):625-628.
• Gürel, S. e Y. Gülsen. 1998. The effects of different sucrose, agar and pH level on in vitro shoot production of Almond (Amygdalus communis L.). Tr. Journal of Botany 22:363-373.
• Gutiérrez E., M. A. 1996. Memorias IV Simposium Internacional sobre Sistemas de Producción de Cítricos. Universidad Autónoma Chapingo, Vol.1. 28-3 de Octubre. Tuxpan, Ver., México. 1:95-98.
• Kataoka, I. 1994. Influence of rooting substrates on the morphology of papaya root formed in vitro. Japan Journal Tropical Agronomy 38(3):251-257.
• Kobayashi, A. K., J. C Bespalhok, L. F .P. Pereira and L. G. E. Viera. 2003. Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments. Plant Cell Tissue and Organ Culture. 74(1):99-102.
• Martínez-Hernández, M. D. J., L. A. Alonso, F. Osorio- Acosta, L. F. Gallardo, M. H. López y R. M. Mata. 2006. Cultivo in vitro de patrones de cítricos tole- rantes al virus de la tristeza, empleando sustratos inertes alternativos al agar. Interciencia. 31(8):616-619.
• Medina-Urrutia, V. M. y J. Valdez-Verduzco. 1990. Crecimiento y producción de limón Bears (Citrus latifolia Tanaka) sobre ocho portainjertos en dos condiciones de suelo. In: XIII Congreso Nacional de Fitogenética. 167 p.
• Murashige, T. and F. Skoog. 1962. A revised médium for rapid grow and biossays with tobacco tissue cultures. Physiology Plantarum. 15:473-497.
• Murcia, R. N., A. J. Osorio, A. Caicedo, L. Calvert y F. Morales. 2002. Distribución y caracterización serológicas de aislamiento del virus de la tristeza de los cítricos en Colombia. Fitopatología Colombiana. 26:21-26.
• Orozco-Santos, M. 1996. Enfermedades presentes y potenciales de los cítricos en México. Universidad Autónoma Chapingo, México. 150 p.
• Padrón J., E. 2000. Precauciones y uso de patrones cítricos tolerantes a tristeza. Sociedad Mexicana de Ciencias Hortícolas A.C.P. pp.36-43.
• Pio, R., J. D. Ramos, L. A. S. PIO, V. Mendonca, A. B. Silva and M. Pasqual. 2002. In vitro rooting of sprouts of Tangerina sunki x Trifoliata english 63-256 citrus rootstocks through the use of sucrose and indolebutyric acid. Ciéncia e Agrotecnologia. 26(1)66-70.
• Ruzéicå, D., M. Saricå, R. Cerovicå and L. Cåulaficå. 2000. Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro. Plant Cell, Tiss. Organ Cult. 63:9-14.
• Secretaría de Agricultura, Ganadería, Desarrollo rural, Pesca y Alimentación (SAGARPA. 2007. Sembrando soluciones. Secretaria de Agricultura, Ganadería, desarrollo Rural, Pesca y Alimentación.https://bit.ly/3hYbLnc. Consultado el 11 de Marzo de 2007.
• SAS Institute Inc. 1997. SAS/STAT User´guide, versión 6.12. University of Minnesota.
• Simoes M. O. M., E. A. M. Da Silva, L.Teixeira e P. R. Cecom. 1990. Obtencao de brotacoes adventicias de Citrus sinensis (L). Osbek de regioes isoladas de internódios em diferentes estádios de desenvolvi- miento. Resvista Ceres 37:337-344.
• Singh I., P. 2002. Micropropagation in citrus . Agricultural Reviews 23(1):1-13.
• Slesak, H., A. Skoczowski and L. Przywara. 2004. Exogenous carbohydrate utilization by explants of Brassica napus cultured in vitro. Plant Cell Tissue and Organ Culture. 79:45-5.
• Song M., K. R., G. L. Silva, R. T. Faria e L. S. A.Takahashi. 1999. Análise do crescimento e enraizamento in vitro de híbridos de Dendrobium novile Lindl. (Orchidaceae) semeados em diferentes meios de cultura. In: Congresso Brasileiro de Floricultura e plantas ornamentais. Jaboticabal, Anais. SP, Brasil. p.110.
• Zimmerman, R. H. 1995. Environmental effects and their control in plant tissue culture Overview. Acta Horticultura e 393:11-13.
Published
2009-09-30
How to Cite
Martínez-Hernández, M. de J., López, A. A., Osorio-Acosta, F., Gallardo López, F., López Moctezuma, H., & Mata Rosas, M. (2009). Evaluation of different sources of carbohydrates and medium of support for the in vitro multiplication of rootstock tolerant to citrus tristeza virus. Agronomía Tropical, 59(3), 343-350. Retrieved from http://publicaciones.inia.gob.ve/index.php/agronomiatropical/article/view/426
Section
Original research article
Copyright (c) 2009 María de Jesús Mertínez-Hernández, Alejandrp Alonso López, Francisco Osorio-Acosta, Felipe Gallardo López, Héctor López Moctezuma, Martín Mata Rosas
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
